Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

Shown for zasosuvannya method

Zhinoce without pay:

  1. Absolutely no-pipe (inconsistency of dull pipes or their neprohidnіst).
  2. Without yplіddya, Yake is charged with endometriosis (with no medication, without medication therapies).
  3. Endocrinne besplіddy (with indispensable hormone therapy).
  4. Free from nezumum ietologii.
  5. Unchecked, obumovlene cervical factor (with unsuccessful lіkuvannі Shlyakh vnutrіshnymatokovo Іnsemіnatsії).
  6. Absolutely free of charge, deducted from a single point of operation or functionally inconsistent I have been, in tsip vykadka ekstrakorporalnaya zaplіdnennya i iponesennya embrіonіv We will include the donor virus donation of oocytes and chambers.

Чоловіче безпліддя:

  1. Oligoasthenozoospermia 1-2 tbsp.

Prototype for zasosuvannya method

  1. Somatic and Psychological Empowerment, Prototype to vinochuvannya vagіnnostі (for visnovkom profіlnogo spetsialіsta).

  2. Vrodzhen anomalies: reappointment of young women with the same type Wadami rozvitku; popular female ditini with chromosome anomalies; dominantly succeed in one бz batkіv z high step pentestrannost_ (behind viskovkom lіkarya - genetics).

  3. Spadkov_ captivity: heterozygosity among friends along all bagatogenous the captured (Porushennya amіnokislotnogo, carbohydrate, glіkіpіdnogo, glycoprotein obmіnіv). Population of children with successful absorption, (hemophilia, Myopathic type of Dyushena and ін.), behind Visnovk lіkarya-genetics.

  4. Puhlinnі і gіpplastichnі stani uterus і iникникnikіv.

  5. Defects rozvitka uterus (for visnovkom lіkarya obstetrician-gynecologist).

  6. Stateteum infantilism (dovzhina t_la uterus less 35 mm).

  7. Synechіі empty womb.

  8. Vіk zhinki more 40 rokіv.

Punktsіya i aspіratsіya vm_styu folіkulіv

Punktsіya held in the operations, equipped with all the neobhіdny іnstrumentarієm і izobljannyam for extrnoo ї medchno ї help, Before the rules of asepsis for a triumphanton, laid on a gynecological count.

Punctica to be held in the city of Ukraine for short-term internal abstinence anesthesia.

Vibe to the method of nobility virushuєtsya indivnuduvalno in the presence of the nature of the vidpovіdі on the stimulation of the superovulation (kіlkostі folіkulіv), psychology camp patsіntki.

 Otrimannya eggllo from aspired by folic ridi

Pislya aspiratsii, odzhra zh, vm_st folikul_v delivered from operatsii to ізолованої laboratories, yak z'єdnana z operatsіynoyu.

Aspirations vm_st folіkulіv look pіd stereolupuyu.

Viyavlenі to transfer oocytes after the help of a pasteurіvska і pіpetki abo special capillary in sterile cups Petr, scool in the middle of the day, yak ekvіlіbrovane in Atmosphere of 5% CO2 and a temperature of 37 degrees C, with a Vologost of 98% with a stitch of 12-20 Godin (taki uvovi zvoryuyutsya special іnalnym automatic CO2 incubator).

In a cup for cultivation, no more than 2-3 eggs will be transported. Posttransfer cups markuyut і znovu vmіschyuyut in incubator for 6-7 years before інсеінації.

All manipulyes with clinins, cups, sredovischami ta іn. be held in a special box with a stream of sterile tracer.

Manіpulyatsії no more than 1 hilini.

Preparation of sperm to іnsemіnatsії, іnsemіnatsіya

Method Semen handling includes such procedures:

  1. Srash sperm doskіnu мtsya mіkroskіchno to the concentration spermatozoa, ikh rukhliv_st, morphology, kіlkіst leukocytes in zrazku.
  2. When normal yakostі zrazka 1 ml of sterile sterile pipetka transferred from the centrifugal probe with 2 ml of an inceptive medium, retractable, centrifuged with 10 killers 3000 (r / h).
  3. A supernatant will be collected with a sterile perfume when centrifuged. Before the pellet give 2 ml of the first іnsemіnatsіynogo srednesischa, Allow 10 khivin centrifugation at 1500 r / h.
  4. The supernatant is tidied up, on the pellet nasharovuyat 1 ml of an inceptive medium, sample taken from the patient is transferred to the automatic CO2 incubator and incubation at 37 degrees C, 5% CO2 and 98% Vologost 40-60 hvilin (for one hour, find the best spermatozoids spill at the top of the ball іnsemіnatsіynogo sredstvischa).
  5. Sterile mіkropіpetkoyu abo deyaka part supernatant climb for doslіdzhennya і concentration and spermatozoa, Scho spilled.
  6. Unnecessary Ob'n іnsemіnatsіynogo sredstvischa come out at the Petrі Cup for cultivation gamete and embryo, in 1 ml of culture medium, there are 1-3 oocytes, Otrimanich at punktsі.
  7. Cultural cup with gametes Get into the incubator for 18-20 years before carrying out the offensive steps of cultivation. Warehouse middles, yakі vikoristovuyutsya for vіdmivannya spermatozoids and cultivation of gametes, may be the same, or similar, al Ph i osmopolar tsikh sredovische povinni buty tomozh.
  8. Usi manipulace with egg and sperm are carried out at the sterile disposable dishwasher sterile disposable instruments.
  9. Specialist Vidpovіdalnist for markuvannya gamete nembrіolog.

The first day of cultivation and transfer of embryos to empty uterus.

After 18-20 godin pіslya іnsemіnatsії oocyte vivіlyayut vid klіtin cumulus that yogi is a yakost.

Send a message to a keyframe in ooplasm.

Inspiration Klіtin Cumulus robs the nobility of the oocyte with the oocyte clitin through the trochs for the oocyte, I’ll make a sterile microplate for the oocyte, retract over half of

Manіpulyatsії with an oocyte, guilty buti duzhe oberezhny, schob not zruynuvati, not uskititi oocyte.

Manіpulyatsії lead to the Victorian stereomikoskopa (lupi).

Pislya manipulace oocyte is transferred to the Petri dish with a cultural medium for crushed that rozbitka embrіona.

Independence two pronucleus about normal zapl_dnennya. Zygots, in ooplasm akih pronuclei of vsdsutnі abo їх bіlshe two, not guilty for the cultivation of cultivators.

Cultural cups mark with the help of children, kylkisty zygotes, they should be in them, that their yakistyu.

Cups іz zygotes are transferred to the incubator for the far-off cultivation.

Offensive days pіslya punktsі ї (vranci і) zygoti mikrokopuyusya in the face of crushed.

Embrioni, yaki split up to reach the cluster of blastomeres, іх рівномірність, homogenousness cytoplasm abo on the manifest fragmentation.

Embryony, yakі vіdіbranі, transferred to one culture bowl і filled in in the incubator until the moment of transfer.

Potim embrion transferred zhіntsі s empty uterus.

Method of corporal recording and transferring of the embryo (embryo) in the empty uterus images, pictures

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  • Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus

    Method of corporal recording and transferring of the embryo (embryo) in the empty uterus